Antimalarial assessment of Viola odorata L. by heme polymerization inhibition method
Authors
Abstract:
Background and objectives: Viola odorata L. is a grassy plant that grows in the humid regions and has been used to treat liver diseases and complicated and tropical fevers in Iranian traditional medicine (ITM). V. odorata has cold and humid temperament while the temperament of fever which is the most important symptom of malaria is the opposite. Antimalarial activity of the petroleum ether fraction of V. websteri has been reported through pLDH method in vitro. In this study, the antimalarial effect of V. odorata which has been used for the treatment of fever in ITM has been evaluated by heme polymerization inhibition method. Methods: The fractions were prepared through maceration during four consecutive days using petroleum ether, chloroform, methanol and water; each day the mixture was filtered and the next fresh solvent was added to the dry plant residue. The heme polymerization inhibition method was carried out in 96-wells plate at the concentration of 200 μg/mL for each sample. The absorbance was recorded at 405 nm with an ELISA reader and the heme polymerization inhibition was determined. Results: The results demonstrated that the petroleum ether, chloroform, methanol and aqueous fractions of V. odorata inhibited heme polymerization up to 62, 77, 100and 53%, respectively while the methanol fraction of V. odorata showed the most considerable results. Conclusion: The results introduced the methanol fraction of V. odorata L. as a proper candidate for further antimalarial studies.
similar resources
Inhibition of Heme Polymerization, the Mechanism of Antimalarial Activity in Phlomis caucasica Rech.f. (Lamiaceae)
Background: Malaria is one of the most important parasitic diseases in the world caused by Plasmodium species. The malaria parasite digests hemoglobin in vacuole to amino acids and heme. Plasmodium has got several detoxification mechanisms to protect itself from toxic heme. The most important mechanism is heme polymerization. Identifying compounds that inhibit heme polymerization is an approach...
full textPhytochemical and Pharmacological Potential of Viola Odorata
Sweet violet also known as the Viola odorata, blooms in continental climate conditions in early spring with delicate flowers of attractive scent. It has been traditionally the part of various indigenous preparations and being used to cure respiratory and inflammatory conditions. Present review article is an attempt to cover recent developments in phytochemical and pharmacological potential of d...
full textInhibition test of heme detoxification (ITHD) as an approach for detecting antimalarial agents in medicinal plants
Background and objectives: There are several methods to assess the in vitro capability of heme inhibitory activity of antimalarial compounds; most of them require some specific equipment or toxic substances and sometimes the needed materials are not accessible. Regarding the necessity and importance of optimizing and standardizing experimental conditions, the present s...
full textRelationship between antimalarial drug activity, accumulation, and inhibition of heme polymerization in Plasmodium falciparum in vitro.
We have investigated the contribution of drug accumulation and inhibition of heme polymerization to the in vitro activities of a series of antimalarial drugs. Only those compounds exhibiting structural relatedness to the quinolines inhibited heme polymerization. We could find no direct correlation between in vitro activity against chloroquine-susceptible or chloroquine-resistant isolates and ei...
full textEfficacy of Viola odorata in Treatment of Chronic Insomnia
BACKGROUND Insomnia is the most common sleep disorder that reduces quality of life. OBJECTIVES Due to side effects of hypnotic drug and the increasing demand for alternative medicine substitutes, violet oil (VO) was used in this study. VO is a known medication in Iranian traditional medicine that induces sleep in insomniac patients. PATIENTS AND METHODS This study was conducted as an experi...
full textMy Resources
Journal title
volume 4 issue Supplement
pages 32- 32
publication date 2017-11-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023